Acta Pharm. 48 (1998) 85-92

Original scientific paper  

Optimisation of thin-layer chromatographic analysis of flavonoids and phenolic acids of Salviae folium*


1Department of Pharmacognosy, 2Department of Pharmaceutical Chemistry,
Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia 
Received February 27, 1998      Accepted May 6, 1998

The information content and values of discriminating power have been calculated for 11 systems used in thin-layer chromatographic (TLC) investigations of flavonoids and phenolic acids of Salviae folium. The TLC systems were classified by clustering methods. The results obtained by these numerical methods are similar. Most suitable chromatographic systems for the separation of investigated compounds are: ethyl acetate - formic acid - acetic acid - water (100:11:11:27 V/V) and ethyl acetate - formic acid - water (8:1:1 V/V).

Keywords: Salviae folium, thin-layer chromatography, flavonoids, phenolic acids, numerical methods, clustering